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p 2000 laser based micro pipette puller  (Sutter Instrument Company)


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    Structured Review

    Sutter Instrument Company p 2000 laser based micro pipette puller
    P 2000 Laser Based Micro Pipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-2000+laser-based+pipette+puller/P-2000%2FG+Laser-Based+Glass+Puller/pm40716064-550-19-23
    Average 93 stars, based on 49 article reviews
    p 2000 laser based micro pipette puller - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Nestin-expressing cells divide and adopt a complex electrophysiologic phenotype after transient brain ischemia.
    Article Snippet: Patch pipettes were pulled from borosilicate capillaries (inner diameter 0.87 mm; outer diameter 1.5 mm; Hilgenberg, Malsfeld, Germany) using a P-2000 laser-based pipette puller (Sutter Instrument, Novato, USA).

    Article Title: Nanophysiology: Bridging synapse ultrastructure, biology, and physiology using scanning ion conductance microscopy.
    Article Snippet: 2 Synaptic communication is at the core of neural circuit function, and its plasticity allows the nervous system to adapt to the changes in its environment.. Understanding the mechanisms of this synaptic (re)organization will benefit from novel methodologies that enable simultaneous study of synaptic ultrastructure, biology and physiology in identified circuits.. Here we describe one of these methodologies, i.e. Scanning Ion Conductance Microscopy (SICM), for electrical mapping of the membrane anatomy in tens of nanometers resolution in living neurons.

    Article Title: A subpopulation of precursor cells in the mouse dentate gyrus receives synaptic GABAergic input.
    Article Snippet: Fate choice decision and the initiation of neuronal development occur in precursor cells of the adult dentate gyrus.. We here show that the earliest signs of neuronal function can be detected in a nestin-positive progenitor cell population, which is morphologically distinct from the putative radial glia-like stem cells.. Whereas radial glia-like stem cells had a glial physiological phenotype, this other cell population was more heterogeneous including cells with the ability to generate action potentials.

    Article Title: GABAergic activities enhance macrophage inflammatory protein-1α release from microglia (brain macrophages) in postnatal mouse brain
    Article Snippet: Patch pipettes were pulled from borosilicate capillaries (inner diameter 0.87 mm; outer diameter 1.5 mm; Hilgenberg, Malsfeld, Germany) using a P-2000 laser-based pipette puller (Sutter Instrument Co., Novato, CA, USA) and filled with pipette solution containing (in m m ): KCl 130; MgCl 2 2; CaCl 2 0.5; Hepes 10; EGTA 5 at pH 7.3.

    Article Title: Analyte detection method
    Article Snippet: Nanopipettes were fabricated using a P-2000 laser-based pipette puller (Sutter Instrument, US).

    Article Title: Small molecule electro-optical binding assay using nanopores.
    Article Snippet: In brief, capillaries (internal diameter: 0.5 mm, external diameter: 1.0 mm, length: 7.5 cm) were plasma cleaned for 10 min to remove any contaminated residues and pulled using a P-2000 laser-based pipette puller (Sutter Instrument, USA) to achieve two nanopipettes under the set of a two-line pulling protocol: (1) HEAT: 775; FIL: 4; VEL: 30; DEL: 170; PUL: 80, (2) HEAT: 825; FIL: 3; VEL: 20; DEL: 145; PUL: 180.

    Article Title: Biological nanopores elucidate the differences between isomers of mercaptobenzoic-capped gold clusters.
    Article Snippet: The pipette was formed using preset program #11 on a P-2000 laser-based pipette puller (Sutter Instruments, Novato, CA) to obtain a final ID of 1–2 mm.

    Article Title: Single molecule multiplexed nanopore protein screening in human serum using aptamer modified DNA carriers.
    Article Snippet: Nanopipettes were fabricated using a P-2000 laser-based pipette puller (Sutter Instrument, USA).

    Transferring:

    Article Title: Nestin-expressing cells divide and adopt a complex electrophysiologic phenotype after transient brain ischemia.
    Article Snippet: Patch pipettes were pulled from borosilicate capillaries (inner diameter 0.87 mm; outer diameter 1.5 mm; Hilgenberg, Malsfeld, Germany) using a P-2000 laser-based pipette puller (Sutter Instrument, Novato, USA).

    Article Title: Nanophysiology: Bridging synapse ultrastructure, biology, and physiology using scanning ion conductance microscopy.
    Article Snippet: 2 Synaptic communication is at the core of neural circuit function, and its plasticity allows the nervous system to adapt to the changes in its environment.. Understanding the mechanisms of this synaptic (re)organization will benefit from novel methodologies that enable simultaneous study of synaptic ultrastructure, biology and physiology in identified circuits.. Here we describe one of these methodologies, i.e. Scanning Ion Conductance Microscopy (SICM), for electrical mapping of the membrane anatomy in tens of nanometers resolution in living neurons.

    Article Title: A subpopulation of precursor cells in the mouse dentate gyrus receives synaptic GABAergic input.
    Article Snippet: Fate choice decision and the initiation of neuronal development occur in precursor cells of the adult dentate gyrus.. We here show that the earliest signs of neuronal function can be detected in a nestin-positive progenitor cell population, which is morphologically distinct from the putative radial glia-like stem cells.. Whereas radial glia-like stem cells had a glial physiological phenotype, this other cell population was more heterogeneous including cells with the ability to generate action potentials.

    Article Title: GABAergic activities enhance macrophage inflammatory protein-1α release from microglia (brain macrophages) in postnatal mouse brain
    Article Snippet: Patch pipettes were pulled from borosilicate capillaries (inner diameter 0.87 mm; outer diameter 1.5 mm; Hilgenberg, Malsfeld, Germany) using a P-2000 laser-based pipette puller (Sutter Instrument Co., Novato, CA, USA) and filled with pipette solution containing (in m m ): KCl 130; MgCl 2 2; CaCl 2 0.5; Hepes 10; EGTA 5 at pH 7.3.

    Article Title: Analyte detection method
    Article Snippet: Nanopipettes were fabricated using a P-2000 laser-based pipette puller (Sutter Instrument, US).

    Article Title: Small molecule electro-optical binding assay using nanopores.
    Article Snippet: In brief, capillaries (internal diameter: 0.5 mm, external diameter: 1.0 mm, length: 7.5 cm) were plasma cleaned for 10 min to remove any contaminated residues and pulled using a P-2000 laser-based pipette puller (Sutter Instrument, USA) to achieve two nanopipettes under the set of a two-line pulling protocol: (1) HEAT: 775; FIL: 4; VEL: 30; DEL: 170; PUL: 80, (2) HEAT: 825; FIL: 3; VEL: 20; DEL: 145; PUL: 180.

    Article Title: Biological nanopores elucidate the differences between isomers of mercaptobenzoic-capped gold clusters.
    Article Snippet: The pipette was formed using preset program #11 on a P-2000 laser-based pipette puller (Sutter Instruments, Novato, CA) to obtain a final ID of 1–2 mm.

    Article Title: Single molecule multiplexed nanopore protein screening in human serum using aptamer modified DNA carriers.
    Article Snippet: Nanopipettes were fabricated using a P-2000 laser-based pipette puller (Sutter Instrument, USA).



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